Hepatitis C virus in blood samples from volunteer donors.
نویسندگان
چکیده
The specificities of four assays for hepatitis C virus (HCV) were compared by using units from volunteer blood donors. Upon Food and Drug Administration licensure of the first immunoassay for anti-HCV, EIA-1, units previously deemed acceptable for transfusion and all subsequent blood donations were screened. EIA-1 repeat-reactive (RR) units were tested for HCV by a second-generation enzyme-linked immunoassay (EIA-2) and by a four-antigen recombinant immunoblot assay (RIBA II) and for HCV RNA by reverse transcriptase polymerase chain reaction. All HCV RNA-positive samples were reactive by both RIBA II and EIA-2. All RIBA II-reactive sera were EIA-2 RR. In EIA-1, 0.45% of the prescreened units and 0.59% of the prospectively screened donors were RR. Of these, 52.5 and 54%, respectively, were EIA-2 RR, 71.4 and 69% of the EIA-2 RR units were reactive on RIBA II, and 93 and 88% of the RIBA II-reactive samples were HCV RNA positive. When the sample/cutoff ratio for EIA-2 was greater than 5, 91% of the samples were RIBA II reactive and 82% of the samples were HCV RNA positive. None of EIA-2 RR units with a sample/cutoff ratio of < 5 was RIBA II reactive or HCV RNA positive. In conclusion, RIBA II and RT PCR results are highly concordant. A sample/cutoff ratio of > 5 in EIA-2 is a good discriminator for the likelihood of a true HCV infection on the basis of RT PCR and RIBA II assays.
منابع مشابه
Frequency of Torque Teno Mini Virus in Hepatitis B and C Patients and Healthy Blood Donors in Isfahan, Iran
Background: Recently, some new viruses have been identified for their association with hepatitis which Torque Teno Mini Virus being among them. The aim of this study was to determine the frequency of Torque Teno Mini Virus in healthy individuals and hepatitis B and C patients in Isfahan, Iran. Materials and Methods: One hundred serum samples of healthy individuals from Isfahan Blood Transfusi...
متن کاملPrevalence of HIV, hepatitis B and C infections among volunteer blood donors at the blood transfusion center of Ilam city, Iran
Introduction: Blood derived products have been known as an effective treatment for many years. However, this treatment is not without risk of infections transmission including hepatitis B virus (HBV) and hepatitis C virus (HCV) and human immune-deficiency virus (HIV) in people who received the blood. Nevertheless, due to a high risk of blood born diseases through blood transfusion, screening ...
متن کاملPrevalence and Trends of Transfusion-Transmissible Infections and Study of Confidential Unit Exclusion among Blood Donors in Kurdistan Province of Iran
Background and Aims: Evaluation of the prevalence of transfusion-transmissible infections (TTIs) in blood donors is a valuable index of donor selection and blood safety. This study was conducted to explore the prevalence and trends of TTIs markers and study of confidential unit exclusion )CUE) option among blood donors in Kurdistan province in the west of Iran. Materials and Methods: We conduc...
متن کاملChanges of miR-199a and miR-196b expression levels in blood donors suffering from asymptomatic hepatitis C
Abstract Background and Objectives Hepatitis C virus is the main cause of hepatitis C infection that usually has no symptoms at early stages. Studies have shown changes in the expression level of microRNAs in various diseases such as hepatitis. MiR-199a and miR-196b have an inhibitory effect on hepatitis C virus. In a recent study, we examined the expression levels of miR-199a and miR-196b in ...
متن کاملMolecular detection of hepatitis delta virus in blood donors with RT-PCR
Abstract Background and Objective: Hepatitis delta virus is an imperfect virus with RNA and its activity depends on the presence of hepatitis B virus. This virus can lead to acute and chronic diseases in the liver. This study aimed to detect the hepatitis delta virus in blood donors with positive Hepatitis B Surface Antigens (HBsAg). Material and Methods: In this Study, 350 serum sa...
متن کاملHepatitis C virus infection and genotypes in blood donors
Abstract: Genotyping of the hepatitis C virus (HCV) is important for designing therapeutic strategies and regional specific diagnostic assays. The aim of this study was to identify the HCV genotypes in HCV infected blood donors. This is the first report on HCV genotypes in blood donors in Iran. In this cross-sectional study, 103 blood donors with hepatitis C were investigated for HCV genotype...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 31 3 شماره
صفحات -
تاریخ انتشار 1993